An Overview on Mammalian Cell Protein Expression: Biopharmaceutical Production

Abstract

Along with its ability to perform post-translational modifications and assemble human protein-like molecular structures, mammalian cell expression has become the most popular recombinant protein production method for therapeutic purposes. While expression and manufacturing have been fully developed, and Chinese hamster ovary cells would be used in the majority of devices on the market and in clinical development, significant progress has been reported in the last several years in developing and engineering new cell lines, as well as introducing novel genetic mechanisms in expression, gene silencing, and gene targeting. More importance has been given to product quality, particularly glycol profile, as a result of the newest analytical techniques development, which leads to a better understanding of the effect of cells were grown during production. Furthermore, the transient gene expression technology platform is particularly essential in the early phases of biopharmaceutical development. The emphasis of this study was on the most recent developments in the field, particularly in active areas such expression systems, glycosylation impact factors, and transient cell proliferation.