Effect of Shaking on Multiplication and Growth of Shoot Cultures in Sugarcane

  • Swapanil Yadav

Abstract

Abstract: An experiment was conducted to study the effect of shaking of cultures on shoot multiplication and growth. Five sets each of 20 bottles, containing shoot cultures of sugarcane varieties CoSe 01235 and CoS 99259 on liquid MS medium were made. Four sets of each variety were kept on separate illuminated rotary shakers and agitated at various speeds (i.e. 60, 90, 120 and 150 RPM) for initial 7 days. The 5th set of cultures containing liquid medium was kept stationary on the growth racks without shaking. The data on number of shoots per culture and shoot length was recorded 15 days after incubation. The results revealed that shaking of cultures enhanced the rate of multiplication in terms of number of shoots per culture, shoot length and shoot vigor. In variety CoSe 01235, maximum 28.3 shoots per culture could be counted in bottles shaken at 120 RPM followed by 25.7 shoots per culture in bottles shaken at 90 RPM. The mean shoot length in cultures shaken at 120 rpm was highest (6.2 cm) as compared to the cultures kept stationary (5.4 cm). Almost similar trend was observed in case of sugarcane variety CoS 99259 which produced the highest number of shoots (31.1) with maximum average length (6.4 cm) in cultures shaken at 120 rpm. The shoots produced in cultures shaken at 120 RPM were vigorous than those receiving other treatments in both the test varieties. On comparison of responses in both varieties, it was found that CoS 99259 showed better responses regarding rate of shoot multiplication and shoot growth as compared to CoSe 01235, irrespective of shaking treatments.

 Keywords: Shaking cultures, stationary cultures, sugarcane, multiplication rate, tissue culture.

Published
2019-11-11
Section
Articles