Evaluation of In vitro antiinflammatory activity of Marsilea quadrifolia Linn. Running title: Antiinflammatory potential of MQ

Abstract

Marsilea quadrifolia (MQ) Linn is a pteridophyte plant belonging to family Marsileaceae commonly known as European water cloves. Methanol extract of leaf of Marsilea quadrifolia was investigated for its anti-inflammatory activity by in-vitro methods. In-vitro anti-inflammatory activity was assessed using albumin denaturation assay, proteinase inhibitory action, membrane stabilization and antilipoxygenase activity at dissimilar concentrations.  Aspirin was used as normal drug. The results showed that Marsilea quadrifolia leaf extract at a concentration range of 100 500 g/ml significant (p<0.01) protects the heat induced protein denaturation. At the concentration of 500 g/ml, significant (p<0.01) inhibition of 51% of protease inhibitory action. Heat induced haemolysis, hypotonicity induced haemolysis and lipo-oxygenase activity were significanly (p<0.01) inhibited at the concentration of 500 g/ml. The findings of the present study suggest that the methanol extract of Marsilea quadrifolia leaf showed significant anti-inflammatory activity