Characterization of Various Concentrations Of Different Sources On Cellulase Production By Bacillus Altitudinis And Bacillus Licheniformis

Abstract

To isolate and characterize the cellulolytic microbes in the gastro intestinal tract of estuarine fish, Etroplus suratensis. Among the 20 screened isolates, five of them showed cellulolytic activity. Of these, the dominant cellulose producers such as Bacillus altitudinis and Bacillus licheniformis were further identified by sequencing of the amplified 16S rRNA gene. Then suitable nutrient sources such as carbon, nitrogen, phosphate, metal ions and surfactants for maximum cellulase production by the chosen two strains were investigated. Among the nine different carbon sources, the maximum value was observed in 3% concentration of fructose for B.altitudinis and in B.licheniformis, maximum value was noticed in 3% CMC. Addition of 12 nitrogen sources, the maximum cellulase was detected at 1% concentration of ammonium hydrogen carbonate for B.altitudinis and 1.50% concentration of ammonium sulphate for B.licheniformis. Upon 7 different phosphate source treatments, the cellulase enzyme production was found to be maximum at 0.1% and 0.5% concentration of sodium dihydrogen ortho pophate for B.altitudinis and B.licheniformis respectively. Among the seven different surfactants, the maximum value was observed ay 0.5% concentration of tritonx100 supplemented medium for both B.altitudinis and B.licheniformis respectively. In nine different metal ions, te maximum cellulase was detected at 1% concentration of magnesium sulphate for B.altitudinis and 1.5% concentration of manganous sulphate for B.lichenifirmis.