Successive Extraction And Preliminary Phytochemical Testing of Different Extracts of Lepidium Sativum Linn. Seeds

  • Chetna Baregama, Dr. Anju Goyal

Abstract

Plant based medicines as the major remedy in traditional system of medicine have been used in
medical practices since ancient times. Phytoconstituents are the biological active compounds
that are present in plants. These phytoconstituents derived from different parts of plants such as
leaves, barks, seed, seed coat, flowers, roots and pulps and thereby used as sources of direct
medicinal agents. Phytochemistry describes the large number of secondary metabolic
compounds present in the plants. The objective of the present study was extraction and
investigation of the presence of various phytoconstituents from the n-hexane, chloroform, ethyl
acetate and methanolic extracts of Lepidium sativum Linn. seeds. Serial exhaustive extraction of
seeds with n-hexane, chloroform, ethyl acetate and methanol was done by using soxhlet
apparatus. Extracts from different solvents were subjected to various phytochemical tests to
detect phytoconstituents like alkaloids, amino acids, flavonoids, tannins, proteins, steroids,
terpenoids, carbohydrates and glycosides. Screening of n-hexane extract indicated the presence
of amino acid, alkaloids, proteins, carbohydrates, terpenoid and saponins, chloroform extract
indicated presence of alkaloids, proteins, carbohydrate, glycoside, triterpenoids and steroids.
Ethyl acetate extract indicated presence of alkaloids, amino acid, flavonoids, proteins,
carbohydrates, steroids and terpenoids, methanol extract indicated presence of alkaloid, amino
acid, tannin, protein, terpenoid, carbohydrate, flavonoids and saponins. This information can be
used further as identification of specific phytoconstituents responsible for particular
pharmacological activity.

Published
2019-10-30
Section
Articles