Physical and Chemical Induced Mutagenesis Study for Identifying Lethality Dose in Andrographis paniculata (Burm.f.) Nees.
Abstract
Andrographis paniculata is a medicinal herb found in tropical region of South and East Asian countries and native in India and Srilanka. The mutation breeding provides more information regarding for the crop improvement through the genetic manipulation. The present study was mainly focused in order to find out the effect of physical mutagens such as Gamma rays and chemical mutagen as EMS. The seeds of A. paniculata treated with different concentration of EMS 5,10,15,20,25,30,35,40,45,50mM and as well as different doses in Gamma ray viz., 5,10,15,20,25,30,35,40,45 and 50KR. The LD50 value was noted at EMS 20mM and 30KR in Gamma ray. Accordingly to results of LD50 value compared to the maximum reduction of seed germination in 30KR and 20mM of EMS treatment. The seedling survival also improved with in decreased below 50 per cent observed in 30KR of gamma rays treatment and in EMS the seedling survival improved with in decreased below 50 per cent observed in 20mM were analyzed in all the different dose of gamma ray and Ems treatments.
Keywords: Andrographis paniculata, EMS, Gamma rays, LD50, Seed germination.