Simultaneous Analytical Validation of Stability Indicating RP-HPLC for Perindopril–Amlodipine Besylate

  • Vimal Kumar Sharma, Devendra Singh Chahar, CP Mishra, Dr. U S Sharma

Abstract

A simple, precise and accurate RP-HPLC method has been developed and subsequently validated for simultaneous estimation of PerindoprilAmlodipine Besylate from their combination dosage form. PerindoprilAmlodipine Besylate are widely used antihypertensive drugs at present but their analytical methods are very costly and very complex to simplify the methods with increasing sensitivity new methods were developed which are simple, precise, eco-friendly, less time consuming, rapid and fast and economically chief. First standard curve was plotted then the method is validated by using recovery studies, linearity, correctness and reproducibility, robustness, ruggedness, detection limit, quantification limits, stability studies etc. The validated technique has been with success used for stress testing analysis of PerindoprilAmlodipine Besylate. The stress testing studies revealed that the tactic was with success utilized to resolve the degraded product from the sample. From the peak purity profile it had been demonstrated that there was no interference of degradation product and the purity of angle were found to be but the purity of threshold. This work was undertaken with an aim of developing HPLC and Specrophotometric techniques for analysis of PerindoprilAmlodipine Besylate. Number of trials was taken for selection of column and M. Phase. The proposed method was validated as per the ICH and USP guidelines.

 Keywords: PerindoprilAmlodipine Besylate, RP-HPLC method; tablet dosage forms.

Published
2019-12-10
Section
Articles