Assessment of Neutralization of Alkaline Cement Industrial Wastewater Through Novel Isolate Bacillus Halodurans C-125
Abstract
Alkaliphilic bacterial strains were investigated in the present study for their capability of reducing pH and alkalinity of wastewater from cement manufacturing industry. Two strains (S1 and S2) were isolated, in the current study. Bacterial strain S1 was found capable for reduction of pH samples from 12.23 to 6.29 and alkalinity reduction in waste water samples from 166 mg/L to 26 mg/L within 72 hours. It was found to be growing in anaerobic condition at wide range of temperatures (10-40o C), NaCl concentration (0-5%) and pH (7-13). Acid production was reported when glucose, cellulose and sucrose were used as source of carbohydrate in H1 medium, which was observed due to reduction in pH. The isolate was characterized with 16S rRNA sequencing technique and biochemical characterization. 16S rRNA gene sequence analysis shows similarity matching (99%) with the sequence of Bacillus halodurans C-125. The presence and absence of external carbon sources efficiently neutralized the high alkaline pH of wastewater. This efficient tool of science can be utilized as an alternative to the traditional acid neutralization method for treatment of alkaline effluent from industries.